Mirror Bisulfite Sequencing: A Method for Single-Base Resolution of Hydroxymethylcytosine
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چکیده
منابع مشابه
Mirror bisulfite sequencing – a method for single-base resolution of hydroxymethylcytosine
CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. ABSTRACT While the role of 5-methylcytosine has been well studied, the biological role of 5-hydroxymethylcytosine still remains unclear due to the limited methods available for single-base detection of 5-hydroxymethylcytosine (5hmC). Here...
متن کاملA Novel method for the simultaneous identification of methylcytosine and hydroxymethylcytosine at a single base resolution
Since the discovery of oxidative demethylation of methylcytosine (mC) by Tet enzymes, an analytical method has been urgently needed that would enable the identification of mC and hydroxymethylcytosine (hmC) at the single base resolution level, because their roles in gene regulation are quite different from each other. However, the bisulfite sequencing method, the gold standard for DNA methylati...
متن کاملQuantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution.
5-Methylcytosine can be converted to 5-hydroxymethylcytosine (5hmC) in mammalian DNA by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing (oxBS-Seq), the first method for quantitative mapping of 5hmC in genomic DNA at single-nucleotide resolution. Selective chemical oxidation of 5hmC to 5-formylcytosine (5fC) enables bisulfite conversion of 5fC to uracil. W...
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BACKGROUND Detection of DNA methylome at single-base resolution is a significant challenge but promises to shed considerable light on human disease etiology. Current technologies could not detect DNA methylation genome-wide at single-base resolution with small amount of sequencing data and could not avoid detecting the methylation of repetitive elements which are considered as "junk DNA". MET...
متن کاملThe Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing
BACKGROUND We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons. The methylation status of cytosines is typically assessed by reaction with sodium bisulfite followed by PCR amplification. Reaction with sodium bisulfite promotes cytosine deamination, whereas 5-methylc...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2018
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.8b02832